Sub-Zero may refer to:
- Bi-Han and Kuai Liang's Grandfather, the first of the Lin Kuei to take the codename Sub-Zero.
- Bi-Han, the elder Sub-Zero who was killed by Scorpion in the original Mortal Kombat Tournament. Bi-Han was soon resurrected by Quan Chi as Noob Saibot, a wraith who is the very incarnation of evil.
- Kuai Liang, the younger brother of Bi-Han, who once went by the code name Tundra, has assumed the role of Sub-Zero out of respect for his fallen sibling.